Title: Coloning,Expressing and Sequence Analysis of the Gene Gp1 of 27K Thermophilic Neutral Protease from Bacilius Licheniformis XJT-9503
Abstract:Thermophilic neutral protease genes Gp1 was amplified from Bacillus licheniformis XJT9503 by using PCR technique. The PCR primers used is designed by N-terminal amino acid sequence and mass spectrum a...Thermophilic neutral protease genes Gp1 was amplified from Bacillus licheniformis XJT9503 by using PCR technique. The PCR primers used is designed by N-terminal amino acid sequence and mass spectrum analysis of the 27K protease from the Bacillus licheniformis XJT9503. The gene was contained 1129 nucleotide with 376 amino acids. The amplified DNA fragments were inserted into an E.coli vector pET-28a to yield the recombinant secrete plasmid p-Gp1 . The Gp1 in pET-28 was expressed in E.coli BL21.SDS-PAGE analysis showed that the molecular weight of the expressed recombinant products was about 27.0 10~(3)kda,which was corresponded with prediction by gene sequenc. Neutral protease gene from B.licheniformis XJT9503 was compared with that of B.licheniformis and the result showed that there was 96% identity for the amino acid sequence of the gene with B.licheniformis 6816 SerA protease and B.licheniformis 1411T KerA protease.Read More
Publication Year: 2005
Publication Date: 2005-01-01
Language: en
Type: article
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