Title: Assay of hydroxyl radical produced in Fe~(2+)-H_2O_2 reaction system by spectrophotometry with carminic acid
Abstract: A new system of Fe~(2+)-H_2O_2-carminic acid for determination of hydroxyl radical(·OH)was reported.The Fenton system produced hydroxyl radical by reaction between Fe~(2+) and H_2O_2. After hydroxyl radical was produced, carminic acid was added and then the color was faded. The amount of hydroxyl radical produced in the system was indirectly assayed through absorbance changed at a wavelength of 530 nm (ΔA_(530)) by means of spectrophotometry. The optimal experimental conditions were obtained. The resistance to hydroxyl radical oxidation of seven antioxidants was evaluated. The results were satisfactory.
Publication Year: 2004
Publication Date: 2004-01-01
Language: en
Type: article
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