Title: Phenylfluorone Assay of Hydroxyl Radical Produced by Cu(I)-H_2O_2
Abstract: It was found that yield of Hydroxyl radicals produced in Cu(Ⅰ)-H 2O 2 system was 100 times more than that produced in Fenton reaction. A fluorimetry method for the determination of hydroxyl radicals was developed based on Phenylfluorone-Cu(Ⅰ)-H 2O 2 system. The excitation and emission wavelength were chosen at 500 nm and 420 nm. With measurement of fluorescence intensity, the content of hydroxyl radicals in the solution was successfully determined. The results of clearing experiment and ESR showed that the method was effective and reliable. This sensitive method can be appiled to sieve the hydroxyl radical scavenger.
Publication Year: 2000
Publication Date: 2000-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot