Title: Effects of Tianma Gouteng Decoction on mRNA expression of CaL-α1C and PMCA1 in vascular smooth muscle cells of spontaneously hypertensive rat
Abstract: AIM:To observe the effects of Tianma Gouteng Decoction on mRNA expression of L-Type Calcium Channel α1 C subunit (CaL-α1C) and plasma membrane calcium-ATPase (PMCA1) in vascular smooth muscle cells of spontaneously hypertensive rat (SHR). METHODS:Twelve-week-old SHRs were assigned randomly to 5 groups:group A (treated with Tianma Gouteng Decoction),group B (treated with Tianma Gouteng Decoction excluded concha haliotidis),group C (treated with Nifedipine),group D (treated with concha haliotidis),group E (treated with normal sodium,NS),each group consisted of 9 rats was adminstrated,once a day for 4 weeks and mRNA expression of CaL-α1C and PMCA1 was measured with RT-PCR half-fix-quantify method. RESULTS:After 4-week-treatment,both Tianma Gouteng Decoction,Tianma Gouteng Decoction excluded concha haliotidis and Nifedipine could regulate downward mRNA expression of CaL-α1C in vascular smooth muscle cells and regulate upward mRNA expression of PMCA1 in vascular smooth muscle cells,concha haliotidis has no effect on mRNA expression of CaL-α1C and PMCA1. CONCLUSION:Tianma Gouteng Decoction can regulate downward expression of CaL-α1C,and regulate upward expression of PMCA1 at the same time,and meliorate the Ca 2+ overload in vascular smooth muscle cells with hypertension.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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