Title: Detection of co-infection of Porcine circovirus 2 and high-pathogenic PRRSV and analysis of viral variation
Abstract: The outbreak of an unknown disease among piglets resulted in more than 80% morbidity and 50% mortality in a large-scale pig industry in Shandong province.The co-infection of PCV-2 and high-pathogenic PRRSV were identified according to the clinical symptoms,pathological changes and PCR or RT-PCR detection results.Swine Pasteurella was also identified using the bacteria cultivation,Gram stain,Giemsa stain,and biochemical test.Then the variations were analyzed based on ORF2 gene of PCV-2 and Nsp2 partial gene of PRRSV.The results showed that the nucleotide identity between the isolated PCV-2 strains is 94.2%,and the nucleotide identity between the isolated PRRSV strains is 99.7%.Furthermore,the nucleotide identity between the isolated PRRSV strains and the latest reported viruses is 99%,but from 95.8% to 97.2% with viruses reported in 2006.In conclusion,co-infection of PCV-2 and high-pathogenic PRRSV occurred in this large-scale piggery,and the infected PCV-2 strains were derived from different origins while the infected high-pathogenic PRRSVs were highly homologous but the nucleotide variations occurred when compared to virus strains that reported in 2006.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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