Title: The Effect of Curcumin on Expression of p300 in the Bladder Cancer T24 Cell
Abstract: Objective To investigate the effect of curcumin on express of regulation p300 and mechanism of anticancer in vitro.Methods The bladder T24 cell was treated with curcumin in different concentration. The cell growth inhibition was determined with MTT, the level of p300、c-fos、c-jun mRNA were assayed by reverse transcriptase polymerase chain reaction(RT-PCR),and the expression of acetyl histone H3 and H4、p300、c-fos、c-jun protein were evaluated by western blot.Results Curcumin could significantly inhibit proliferation of T24 cell in a dose and time-dependent manner. The expression of p300、c-fos、c-jun mRNA and protein was totally decreased with increasing concentrations of curcumin. Curcumin can inhibit the expression of acetyl histone H3 and H4 protein in a dose -dependent manner.Conclusion Curcumin can inhibit the expression of p300,which may be its molecular mechanism of anticancer.
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
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