Title: Effect of curcumin on cathepsin D expression of T_(24) cells
Abstract: Objective To investigate the effect and possible mechanisms of curcumin on the cathepsin D expression and cell apoptosis of T24 cells.Methods In the control group,there was not any curcumin added.In the curcumin group,the contents of curcumin added were 1,10,100μmol/L respectively.T24 cells were observed in 12,24 and 48h after curcumin was added.MTT,TUNEL staining,RT-PCR,immunoblot and image analysis technology were used to detect the growth,apoptosis,cathepsin D protein activity and mRNA level of T24 cell.Results In the curcumin group,inhibitor rate and cell apoptosis increased significantly.The activity and mRNA level of cathepsin D decreased significantly.Conclusion Curcumin can inhibit the expression of cathepsin D of T24 cell in the transcriptional and proteinic level.
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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