Title: Construction of carrier of recombinant pcDNA3.1- BMP7 eukaryotic express and its identification
Abstract: Objective To reconstruct recombinant DNA pcDNA3.1-BMP7 eukaryotic expressing vector byamplifying rabbit bone morphogenetic protein-7(BMP7) gene and then cloning into pcDNA3.1. Methods pGEM-3Zf(-)-BMP7 and pBluescript SK(M13-) were digested by HindⅢ and KpnI, and then BMP7 gene was cloned into pBluescriptSK (M13-). Recombinant plasmid was identified respectively by PCR analysis, restriction endonuclease analysis andnucleotide sequencing. Positive recombinant pBlue-BMP7 and pcDNA3.1 vector were digested by NotⅠ KpnⅠ andBMP7 gene was cloned into pcDNA3.1. The recombinant was verified by restriction endonuclease, PCR analysis andsequencing. Results The cloned BMP7 gene was 1.5 kb long, having the same length and sequence of the gene that ratsand human possessed. Conclusion Eukaryotic expressing vector of pcDNA3.1-BMP7 has been constructed successfully.
Publication Year: 2005
Publication Date: 2005-01-01
Language: en
Type: article
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