Title: Cloning of the Gene Encoding Mouse ING4 and Construction of pcDNA3.1(+)-ING4 Eukaryotic Expression Vector
Abstract: Objective To isolate the gene encoding mouse ING4 and construct pcDNA3.1(+)-ING4 recombiant eukaryotic expression plasmid.Methods Using RT-PCR method the cDNA encoding the mouse ING4 was isolated with total RNA extracted from mouse liver tissue.The cDNA fragment was subcloned into the eukaryotic expression vector pcDNA3.1(+).Analysis by PCR、restricting enzyme digestion and DNA sequencing were carried out to demonstrate the sequence of the plasmid.Results RT-PCR product is about 750bp specific fragment;analysis was conducted by restricting enzyme digestion and PCR of pcDNA3.1(+)-ING4 recombiant plasmid showed that results were about 750bp. DNA sequencing revealed that ING4 cloning was successful.Conclusion The gene encoding mouse ING4 and construction of pcDNA3.1(+)- ING4 eukaryotic expression vector are successfully obtained.
Publication Year: 2005
Publication Date: 2005-01-01
Language: en
Type: article
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