Title: Effect of transfecting bcl-2 gene into cardiomyocytes on their hypoxic tolerance in vitro
Abstract: Objective To study whether transfecting bcl 2 gene into cardiomyocytes can improve their hypoxic tolerance in vitro. Methods bcl 2 cDNA containing human full length open reading frame was recombined into EcoR I site of retrovirus vector pLXSN. The plasmids were packed with liposome and transfected into cell line PA317. PA317/s bcl 2 was selected from the transfected cells by its resistance to G418. Cardiomyocytes were infected with high titer recombined virus produced by PA317/s bcl 2. The transfection was identified with in situ hybridization. The growth properties and tolerance to hypoxia of the cardiomyocytes were studied in bcl 2 gene transfected cardiac and non cardiac cells. Results Eukaryotic cell expression plasmid containing bcl 2 gene was constructed and it could increase the survival of cardiomyocytes under hypoxic condition. Conclusion Transfecting bcl 2 gene into cardiacmyocytes can significantly enhance their hypoxic survival rate.
Publication Year: 2000
Publication Date: 2000-01-01
Language: en
Type: article
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