Title: Inhibition of wild-type p53 gene on the growth of cultured rabbit cardiomyocytes
Abstract: Objective To study the inhibition of wild type p53 gene on the growth of cultured cardiomyocytes and the possibility of p53 gene therapy for cardiomegaly.Methods We transfered wild type p53 gene mediated by recombinant adenovirus vector into cardiomyocytes.The efficiency of transfection and the expression were detected by X gal staining and Western blot.Inhibition was verified by MTT, transmission electronic microscope and flow cytometry(FCM).Results p53 gene could be quickly and effectively transfered into the cardiomyocytes.The p53 protein could be detected in the transfered cells.Wild type p53 gene could significantly inhibit growth of cardiomyocytes,the inhibition depended on dosage and time.Result of FCM showed that wild type p53 gene could affect cell cycle and provide G 1 arrest.Conclusions Wild type p53 gene mediated by recombinant adenovirus vector can effectively transfect cultured cardiomyocytes,which can inhibit cultured cardiomyocytes growth.This study provides an important reliance for gene therapy of cardiomegaly.
Publication Year: 2002
Publication Date: 2002-01-01
Language: en
Type: article
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