Title: The Effect of Mimeng Flower Decoction to the Pathological Changes of Retinals in Diabetic Rats Induced by Streptozocin
Abstract:Objective To study the the effect of Mimeng Flower Decoction to the pathological changes of retinals in diabetic rats induced by Streptozocin(STZ).Methods Used diabetic rats induced by streptozocin(ST...Objective To study the the effect of Mimeng Flower Decoction to the pathological changes of retinals in diabetic rats induced by Streptozocin(STZ).Methods Used diabetic rats induced by streptozocin(STZ) as animal model.Used calcium dobesilate as positive control medicine.Mimeng Flower decoction had three different concentration which were low dosage,medium dosage and high dosage.The following studies were made when the animals were administered for 4 months.①To observe the general status of diabetic rats induced by STZ,such as food,urinary production,weight,etc.②Pathological changes of retinals in diabetic rats were observed with hematoxylin and eosin(HE) stained method.③To detect the protein expression of ICAM-1、E-selectin in retinal of diabetic rats induced by STZ.Results ①General status: STZ induced diabetic rats had lasting and stable hyperglycaemia,displaying with the obvious signs of polydipsia,polyphagia,hyperdiuresis and loss of weight.Medium dosage of Mimeng Flower decoction increased the body weight of rats compared with DM model group(P0.05).②Remarkable pathological changes of retinal of diabetic rats were found in DM model group.In medium dosage group,the pathological changes of retinal of diabetic rats were reduced.③The result of immunohistochemistry test showed that the expression of ICAM-1、E-selectin in retinal increased in DM model group compared with blank group(P0.01).Mimeng Flower decoction groups and Haochang group could down regulate the expression of ICAM-1、E-selectin compared with model group(P0.01).In medium dosage group the expression of ICAM-1、E-selectin were reduced compared with Haochang group.Conclusion STZ induced diabetic rat is a simple,easy and reliable animal model.Medium dosage of Mimeng Flower decoction can reduce the the pathological changes of retinal and retinal capillary vessels in diabetic rats to a certain.The effect of improving the pathological changes of retinal and retinal capillary vessels may be contributed to downregulating ICAM-1、E-selectin expression in retinal.Read More
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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