Title: GENERATION AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES AGAINST CHICKEN INTERFERON β
Abstract: Eight-week-old Balb/c mice were immunized with purified recombinant pCold-Ch IFN-β.Spleen cells were collected and fused with myeloma SP2/0 cells.Two hybridomas secreting antibodies against IFN-β were obtained and their ascitic ELISA titers were up to 103.Western blot analysis showed that both McAbs reacted with recombinant IFN-β protein.The minimum detection concentration of one McAb was below 15 pg/mL IFN-β.The generation of specific McAbs could provided an important tool for detection of IFN-β and evaluation of poultry vaccines.
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
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