Title: Determination of Total Tanshinone and TanshinoneIIA in Salvia Miltiorrhiza Bge.f. Alba by HPLC
Abstract: Objective To determine total tanshinone and tanshinoneⅡA in Salvia miltiorrhiza bge.f.alba by HPLC. Methods Ultrasonic extraction with 90% ethanol was used to extract the lipid soluble component in Salvia miltiorrhiza bge.f.alba.The liquid chromatography was carried out by using waters symmetry shield RP18 column(150 mm×3.9 mm,5 μm) with detection wavelength at 270 nm.The mobile phase was 90% methanol in determining the total tanshinone,75% acetonitrile in determining the tanshinoneⅡA.The flow rate was 0.8 mL·min-1. Results There was a good linear relationship within the range of 0.075-0.750 mg·mL-1(total tanshinone) and 7.5-50.0 μg·mL-1(tanshinoneⅡA)(r=0.999 4,n=5). Conclusion The method is simple,rapid,selective and sensitive.Total tanshinone and tanshinoneⅡA are rich in Salvia miltiorrhiza bge.f.alba.
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
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