Title: Technical Studies in Rapid Tissue Culture of Yunzhe 03-194
Abstract: The young leaves of sugarcane Yunzhe 03-194 was used as explants.Different concentration of 2,4-D was added to MS medium to conduct the calli induction of sugarcane young leaves.Different hormone combinations of 6-BA and KT added to MS medium was used for the induction of differentiation and propagation culture.The rooting of plantlets using MS medium with different concentration of NAA and different dosage of banana juice.The result showed that MS+2,4-D 1.5 mg/L was the optimum medium for calli induction,MS+6-BA 1 mg/L +KT 0.5 mg/L was the optimum medium for induction of differentiation,the optimum medium for propagation culture was MS+ 6-BA 2.0 mg/L +KT 1.0 mg/L,the best medium for plantlets rooting was MS+ NAA 1.5 mg/L+30mL banana juice.The regenerated plantlets were the transplanted successfully in mixture soil with river sand : laterite(2:1) with survival rate of 92%-96%,and the plantlets grew well and vigorously.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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