Title: The Effect of TFPI-2 on Apoptosis of Pancreatic Carcinoma Cell Panc-1
Abstract: [Purpose] To investigate the effect of TFPI-2 gene on cell proliferation and apoptosis of human pancreatic cancer cell line Panc-1. [Methods] The growth curve was detected in 3 groups, Panc-1-TFPI-2, Panc-1-V and Panc-1-P.DNA strand breaks was used to detect the apoptosis in the 3 groups. The change of cell cycle and apoptosis index were evaluated by flow cytometry. [Results] Compared with nontransfected cells (Panc-1-P, Panc-1-V), the growth of Panc-1-TFPI-2 cell was slower and inhibited significantly.The transfected cells showed a significant increase in G0~G1 phase.The apoptosis of Panc-1-TFPI-2 cell was identified by DNA strand breaks and flow cytometry in comparison with the control group.The apoptosis index of the Panc-1-TFPI-2 cell (6.93%±0.53%) was higher than that in Panc-1-V (3.01%±0.39%) and Panc-1-P (3.52%±0.41%), with significant difference(P0.05). [Conclusion] The exogenous TFPI-2 gene can inhibit the growth and induce apoptosis of pancreatic cancer cell, which maybe inhibit the cancer cells proliferation.
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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