Title: Research on promoter methylation and expression deletion of RASSFIA in patients with colorectal cancer
Abstract: Objective To detect the RASSF1A gene CpG island methylation status in colorectal cancer,analyze the mRNA expression,and to investigate its relationship with clinicopathological features of colorectal cancer.Methods RASSF1A gene promoter area methylation status were detected by using methylation-specific polymerase chain reaction in 31 cases of colorectal cancer mucosa tissue,29 cases of normal mucosa tissue.The relationship between methylation and clinicopathological features was analyzed statistically.RASSF1A mRNA expression in each group were detected by RT-PCR,The correlation between gene expression level with clinicopathological characteristics of colorectal cancer was analyzed statistically.Results The positive rate of RASSF1A gene CpG island methylation was 67.7%in colorectal cancer group and 0.0%in corresponding control group,and the difference had statistical significance(P 0.01).There were no con-elation between RASSF1A methylation rate and clinicopathological features including age,sex,degree of tumor differentiation,staging and lymph node metastasis(Pall 0.05).In 31 cases of colorectal cancer tissues,there were 6 cases of RASSF1A mRNA expression deletion(19.35%),while in 29 cases of control tissue,RASSF1A mRNA were all expressed.There was statistical significance in the difference of expression level between the two groups(P 0.01).There were no correlation between RASSF1A mRNA expression level and clinicopathological features including age,sex,degree of tumor differentiation.staging and lymph node metastasis(Pall0.05).Conclusion The high methylation rate of RASSF1A in colorectal cancer indicates its closely relationship with colorectal cancer occurrence,it may be an important molecular mechanism in early onset of colorectal cancer.But there is no correlation with age,gender and progression of colorectal cancer.
Publication Year: 2014
Publication Date: 2014-01-01
Language: en
Type: article
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