Title: Study on the quality control of Changyanqing mixture
Abstract: Objective:To establish a method for identification of Rhizoma Coptidis in Changyanqing mixture by thin-layer chromatography(TLC)and determination of berberine hydrochloride and palmatine hydrochloride by high performance liquid chromatography(HPLC).Methods:Identification of palmatine hydrochloride and berberine hydrochloride as the effective constituent of Rhizoma Coptidis in preparation by TLC and determination these two components by RP-HPLC.Results:Both characteristics of the active ingredient of Rhizoma Coptidis could be identified by TLC.The linear range of berberine hydrochloride was within 1.34-42.8 μg·mL-1(r=0.9999),the mean recovery was 99.3% with RSD=0.92%(n=9).The linear range of palmatine hydrochloride was within 1.24-39.8 μg·mL-1(r=0.9999),the mean recovery was 98.6% with RSD=0.82%(n=9).Conclusion:The method is simple,rapid,sensitive and accurate with good repeatability,it can be used as the quality control method for Changyanqing mixture.
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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