Title: Study on methodology for determination of main alkaloids in Rhizoma Coptidis by HPLC
Abstract: Objective To establish an HPLC method to determine the main alkaloids of the Coptidis for Chinese Pharmacopoeia of 2010 edition.Methods The C18 column was used.The mobile phase was CH3CN-50 mmol/L KH2PO4(50∶50)(containing 15 mmol/L SDS and adjusting pH=4.0 with H3PO4) at the flow rate of 0.6 ml/min,the detective wavelength was 345 nm and the column temperature was 30℃.Results The linear ranges of epiberberine hydrochloride,coptisine hydrochloride,palmatine hydrochloride and berberine hydrochloride were at 0.0113-0.7240 μg,0.0218-1.3920 μg,0.0148-0.9440 μg and 0.0719-4.6020 μg respectively.The average recoveries were 98.61%,99.70%,98.26%and100.20%;and RSD were 1.4%,1.3%and1.3%,1.5% respectively.Conclusion The method is simple and accurate,and can be used to determine the contents of epiberberine,coptisine,palmatine,and berberine in the Coptidis.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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