Title: Construction and characterization of △Apa1/△Apa2 mutant of Actinobacillus pleuropneumoniae
Abstract: To further investigate the role of trimeric autotransporter adhesins(TAAs) in Actinobacillus pleuropneumoniae,a mutant of double genes deletion of A.pleuropneumoniae(△Apa1/△Apa2) was generated by homologous recombination utilizing high performance suicide vector system.The characterizations of the △Apa1/△Apa2 mutant and wild-type strains were compared in vitro and in mice.PCR confirmed the loss of TAAs genes in the △Apa1/△Apa2 mutant.The △Apa1/△Apa2 mutant showed growth deficiency in BHI media,rough and irregular surfaces;Bacterial aggregation and biofilm formation were decreased when compared to the wild-type strain.And in vitro adhesion rate,△Apa1/△Apa2 mutant reduced adherence to the RAW264.7 cells.For the mice,the virulence of the △Apa1/△Apa2 mutant was reduced and showed the decrease ability to colonize in mice lungs and spleens,but no obvious difference in the histopathology of mice lungs were observed about different strains.This study illustrates the important role of TAAs in the bacterial growth,cell morphology,autoagglutination,biofilm formation,adherence,ability to colonize in mice.Further suggest a putative role of TAAs in the virulence of Actinobacillus pleuropneumoniae.
Publication Year: 2014
Publication Date: 2014-01-01
Language: en
Type: article
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