Title: Protective effect of crocetin on hydrogen peroxide-induced cell injury
Abstract: Objective To investigate the protective effect of crocetin in H2O2 induced rat pheochromocytoma line PC12,and explore the mechanisms on the injury.Methods The cell viability was detected by Cell Counting Kit-8(CCK-8) assay.Mitochondrial membrane potential(MMP) was stained by the Rhodamine123 and determined by flow cytometry.Reactive oxygen species(ROS) was observed by using DCF-DA staining and photofluorography.Phosphorylation of ERK1/2 was measured by Western blot.Results At the concentrations from 0 to 400 μmol/L for 12 h,the cell viability are(100±4.1)% 、(102±1.9)% 、(89±11.2)% 、(52±2.6)%、(42±1.6)%、(8±0.4)%,respectively.H2O2 dose-dependently inhibited cell viability.Exposure of the cells to different doses of crocetin(0~10 μmol/L) for 24 h followed by subsequent exposure to a single dose of H2O2(200 μmol/L),the cell viability increased from(45.12±3.15)% to(51.88±4.24)%、(65.14±8.19)%、(57.66±5.58)%、(53.61±4.57)%.Preincubation of cells with crocetin(1 μmol/L,5 μmol/L) 24 h prior to H2O2 exposure attenuated the decrease of MMP,scavenged ROS formation and activated ERK1/2 phosphorylation.Conclusion The crocetin holds potential for protective effects against H2O2-induced injury.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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