Title: Construction and Identification of Eukaryotic Expression Vector Expressing Human DC-STAMP
Abstract: Objective To construct an eukaryotic expression vector which expresses human DC-STAMP gene,and to confirm its expression in 293T cells.Methods Human DC-STAMP cDNA was amplified with reverse transcription-polymerase chain reaction(RT-PCR) technique and inserted into eukaryotic expression vector pEGFP-N1-FLAG.The recombinant plasmid was then transfected into 293T cells.The expression of fusion protein was tracked under fluorescence microscope and confirmed by Western blot.Results The full length of human DC-STAMP cDNA was amplified successfully using RT-PCR.The recombinant plasmid pEGFP-DC-STAMP-FLAG was successfully constructed and the expression of fusion protein can be detected in 293T cells.Conclusion DC-STAMP fusion gene expression vector has been successfully constructed.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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Cited By Count: 2
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