Title: [Construction and expression of the eukaryotic green fluorescent protein expression vector pEGFP-N1-ZNF217].
Abstract: To construct the eukaryotic green fluorescent protein expression vector pEGFP-N1-ZNF217 and express the vector in eukaryotic cells.ZNF217 gene fragment was amplified by reverse transcriptase-polymerase chain reaction (RT-PCR), and after analysis of the product by electrophoresis and sequencing, the fragment was inserted into pEGFP-N1 fluorescent expression vector. The constructed expression vector was then transfected into eukaryotic cells for its expression.Restriction endonuclease digestion and sequence analysis confirmed correct construction of the recombinant vector pEGFP-N1 and the expression vector pEGFP-N1-ZNF217, which can be stably expressed in eukaryotic cells.
Publication Year: 2010
Publication Date: 2010-02-01
Language: en
Type: article
Indexed In: ['pubmed']
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