Title: Genetic diversity of endangered wild Cypripedium japonicum populations: An AFLP analysis
Abstract: Cypripedium japonicum is a rare and endangered terrestrial orchid. To explore and formulate the pertinent conversation strategies of this species, this paper analyzed the genetic diversity and genetic structure of six natural C. japonicum populations in Zhejiang Province of East China by the method of AFLP. A total of 377 distinct bands were amplified by 7 pairs of selected primers. The percentage of polymorphic loci was 20.95%, Nei’s genetic diversity index was 0.0512, Shannon information index was 0.0417, genetic differentiation coefficient was 0.5178, and gene flow was 0.4655, indicating that there was a relatively low genetic diversity within the C. japonicum populations, and a certain degree of genetic divergence existed among the populations. The analyses of UPGMA clustering and Mantel test showed that there was no significant correlation between the geographical distance and genetic distance (r=0.16, P=0.242). Some conservation strategies were proposed based on this study and the biological properties of C. japonicum.
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
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Cited By Count: 4
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