Title: Genetic Diversity Analysis of Endangered Plant Camellia pubipetala Detected by ISSR
Abstract:ISSR markers were used to analyze the genetic diversity and genetic structure of 6natural populations of Camellia pubipetala.Eleven primers were used to amplify DNA samples from 150individuals,and a t...ISSR markers were used to analyze the genetic diversity and genetic structure of 6natural populations of Camellia pubipetala.Eleven primers were used to amplify DNA samples from 150individuals,and a total of 92loci were detected.The genetic diversity of C.pubipetalais relatively high both at the species level and population level.At the species level,the percentage of polymorphic bands(PPB)is 80.43%, Nei's gene diversity(h)is 0.245 1,Shannon information index(I)is 0.377 6.At the population level, PPBrange from 58.70%~66.30%,h range from 0.199 7~0.229 3,I range from 0.300 9~0.343 8.A low level of genetic differentiation among populations was detected based on Nei's genetic diversity analysis(Gst =0.126 6)and analysis of molecular variance(Φst =11.37%).Gene flow(Nm)among the populations is high(3.448 0).A mantel test showed there was a significantly correlation between genetic and geographic distance among the populations studied(r=0.755 1,P0.05).Relatively high genetic diversity within the population and the low population differentiation of C.pubipetala might be related to its outcrossing mating system and bird pollination.Read More
Publication Year: 2014
Publication Date: 2014-01-01
Language: en
Type: article
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Cited By Count: 5
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