Title: Effects of Glucose on Development of ICR Mouse Embryos in vitro
Abstract: To study the effects of glucose on the development of ICR mouse embryos
in vitro. Experiment 1: ICR female mice (6-8 weeks of age) were
super-ovulated with i.p. injections of PMSG and hCG, and mated
overnight with males. One-cell embryos were collected at 22-26
hrs after hCG and cultured in CZB supplemented with 0, 0.5, 1.0, 3.0,
5.0 or 10 mmol/L glucose respectively. Experiment 2: One-cell embryos
were collected from the oviducts of ICR female mice, super-ovulated and
cultured in glucose-free CZB. The embryos,respectively at
one-cell, two-cell, four-cell or morula stage,were removed from
glucose-free CZB medium and placed in CZB medium supplemented with 3.0
mmol/L glucose (optimal concentration) and removed to glucose-free CZB
24 h later (except the morula stage embryo). The embryos in another
group were cultured in CZB supplemented with 3.0 mmol/L glucose during
the entire culture time. The embryos in the control group were
continuously cultured in glucose-free CZB. Embryos were cultured for
120 h at 37°C under 5% CO2 in sealed culture chambers and observed
every 24 h under a Nikon inverted microscope. The culture efficiency
was evaluated by determining the proportion of embryos reaching the
four-cell (48 h), blastocyst (96 h), or hatched blastocyst (120 h)
stage. The total cell numbers were counted in the blastocyst and
hatched blastocyst embryos. The experiment results were: 1) There were
no significant differences in the rates of four-cell embryos between
every glucose-containing group and the glucose-free group. 2)The
blastocyst rates of glucose-containing groups were significantly higher
than that of the control. 3)The total cell numbers in the concentration
group containing 3.0 mmol/L glucose were significantly higher than one
another. 4)Addition of glucose from the two-cell to four-cell stage or
from the four-cell to morula stage, significantly increased the
blastocyst rates compared with the control. In contrast, addition of
glucose from one-cell to two-cell, at morula or after morula, did not
increase the blastocyst rates compared with the control. It indicates
that addition of glucose to CZB does not result in two-cell block of
the embryo development, and addition of glucose to CZB in a
concentration as high as 10 mmol/L does not inhibit the development of
ICR mouse one-cell to hatched blastocyst development. The optimal
concentration of glucose added in CZB could be 3.0 mmol/L in the
culture of ICR mouse embryos in vitro. Exposure of embryos to glucose,
beginning at the two-cell and extending to the four-cell stage or
beginning at the four-cell and extending to the morula stage, is
necessary for the development of ICR mouse embryos in vitro.
Publication Year: 2007
Publication Date: 2007-10-22
Language: en
Type: article
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Cited By Count: 3
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