Title: Study on cultural method of mouse early embryos in vitro
Abstract: Objective To seek the appropriate way for the culture of mouse early embryos in vitro.Methods One-cell embryos were collected from the oviducts of ICR female mice,super-ovulated and cultured in glucose-free CZB.The embryos,respectively at two-cell,four-cell or morula stage,were removed from glucose-free CZB medium and placed in CZB medium supplemented with 3.0 mmol/L glucose(optimal concentration).The embryos in the other two groups were cultured in CZB supplemented with 3.0 mmol/L glucose during the entire culture time with or without renewal of medium.The embryos in the control group were continuously cultured in glucose-free CZB.Embryo development was recorded at 24 h,48 h,72 h and 96 h respectively culture.Results(1)Shift embryos from glucose-free CZB to glucose-containing CZB at two-cell or four-cell stage,the blastocyst rates significantly increased compared with the control group.(2)The blastocyst rates of which the embryos were continuously cultured in glucose-containing CZB during the entire culture time with or without renewal of medium,were significantly higher than that of the control group.(3) Shift embryos from glucose-free CZB to glucose-containing CZB at morula stage,the blastocyst rates did not increase compared with the control group.(4)The blastocyst rates,shift embryos from glucose-free CZB to glucose-containing CZB at two-cell or four-cell stage and continuously cultured in glucose-containing CZB during the entire culture time with or without renewal of medium,were 46.5%,38.4%,41.7% and 56.6% respectively,but there were no significant differences among groups.Conclusion Exposure of embryos to glucose,beginning at the two-cell and extending to the morula stage,is necessary for the development of ICR mouse embryos in vitro.Sequential Culture is sufficient,but is not necessarily superior to continuous Culture without renewal of medium.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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