Title: Studies on the biological characteristics of Sclerotium rolfsii strain SC64 causing southern blight on Solidago canadensis L.
Abstract: A southern blight of Canadian goldernrod(Solidago canadensis)was found to be an important disease.The pathogen was identified as Sclerotium rolfsii Sacc.based on morphological characteristics.Factors influencing mycelial growth and sclerotia germination of strain SC64 of Sclerotium rolfsii were studied.The results indicated that the most optimum media for the mycelial growth were modified Richard and potato dextrose agar(PDA),the range of temperature for the mycelium growth was 15-40 ℃,with the optimum at 30 ℃,and the range of pH was 4.0-6.0,with the optimal pH of 5.0.Through carbon sources testing on amended Czapek,sucrose was found to support growth significantly faster than other sources,while lactose was found to provide the slowest growth.Among the 13 nitrogen sources tested,peptone and potassium nitrate gave the best growth whereas urea gave the least growth.The range of temperature for sclerotia germination was 15-40 ℃,with the optimum at 30 ℃.Sclerotia germination reached 100% when incubated on bran substrate with ≥50% moisture.The range of pH for sclerotia germination was 2-10,with 100% germination at 3-9.Hyphal and sclerotia viability was completely lost when hyphal agar plugs and sclerotia were incubated at 45 ℃ 10 minutes and 50 ℃ 10 minutes,respectively.
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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