Title: Relationship between mutated k-ras and biological behavior of colorectal cancer
Abstract: AIM:To investigate mutations of oncogene k-ras in colorectal cancer tissues and the relationship between mutations of k-ras and biological behavior of colorectal carcinoma. METHODS:The specimens of 123 patients with colorectal cancer were collected. Real-time fluorescence quantitative PCR were performed to detect k-ras mutations at codon 12 and codon 13 of exon 1,and the results were analyzed with the corresponding clinical pathological data. RESULTS:Among 123 colorectal cancer cases,point mutations were detected in 53 cases (40.8%),point mutations at codon 12 were found in 42 (34.1%) cases,and 11(8.9%) cases at codon 13. No closely relationship between mutations of k-ras and tumor size,location,invasive depth and differentiation extent was observed. The rate of k-ras mutation in the cases with more invaded lymph nodes was higher than that in the cases without invaded lymph nodes (P0.05),and the rate of k-ras gene mutation in the cases with hepatic metastases was higher than that in no hepatic metastases (P0.05). The rate of k-ras gene mutation was higher in TNM staging Ⅲ/Ⅳ than that in Ⅰ/Ⅱ(P0.05). CONCLUSION:Mutation of oncogene k-ras plays an important role in the carcinogenesis and development of colorectal cancer,and it is closely associated with invaded lymph notes and hepatic metastases,suggesting that mutation of k-ras indicates a poor prognosis.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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