Title: Dynamic effect of dexamethasone on IL-25 mRNA and its expression in the lung tissue of asthmatic mice
Abstract: Objective While the action mechanisms of classic cytokines in asthma receive more attention from researchers,some non-classic cytokines,such as IL-25,also play a certain role.This study was to investigate the changes of IL-25mRNA and IL-25(IL-17E) in bronchial asthma,and further understand the action mechanisms of glucocorticoids in asthma.Methods Clean grade BALB/c mice were randomly divided into three normal groups(after the first and second sensitization and excitation),three asthma groups(after the first and second sensitization and excitation) and a dexamethasone group.Asthma models were established by the ovalbumin-sensitized excitation method.The mice of the dexamethasone group were intraperitoneally injected with dexamethasone 1 hour before each excitation,while those of the normal groups were sensitized and excited with isotonic saline.The IL-25 level in the bronchoalveolar lavage fluid was measured by ELISA,and the relative content of IL-25mRNA in the lung tissue detected by real-time PCR.Results Compared with the asthma groups,both IL-25 and IL-25mRNA were significantly increased(P0.05),but remarkably decreased after the second sensitization(P0.05) and excitation(P0.05) in the normal groups.The dexamethasone group showed significantly lower levels of IL-25 and IL-25mRNA than the asthma group after excitation(P0.05) and a higher level of IL-25 than the normal group after excitation(P0.05),but there was no significant difference in the expression of IL-25mRNA between the dexamethasone and normal group after excitation.Conclusion IL-25 is involved in the whole process of inflammation and inflammatory immune pathogenesis of asthma.One of the mechanisms of glucocorticoid acting on asthma may be its inhibitory effect on the expression of IL-25.
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
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