Title: Effect of exercise on the content of serum plasminogen activator inhibitor type-1 in insulin resistant rats
Abstract: 【Objective】The changes of serum plasminogen activator inhibitor type-1(PAI-1) and the effect of swimming exercise to the content of serum PAI-1 were observed in the high-fat diet-induced insulin resistance rats,in order to explore the possible mechanisms by which the no-drug therapy-exercise improves the insulin resistance.【Methods】Male Wistar rats(4 weeks old,70-80 grams)were randomly assigned to normal control group(group NC) and high-fat-diet feed group(group HF).10 weeks later,group HF was randomly assigned to high-fat-diet feed with exercise group(group HFE) and high-fat-diet feed without exercise group(HFNE),then the HFE groups were arranged to swim for 4 weeks after insulin resistant rats model was established by high-fat diet feeding.The changes of insulin sensitivity after exercise were assessed by euglycemic hyperinsulinemic clamp technique.The serum PAI-1 was measured by ELISA method.The mesentery fat tissue and paraepididymal adipose tissue were weighed at last.SPSS 16.0 was used to analyze all the data.【Results】The glucose infusion rate60-120(GIR60-120) in group HF was significantly lower than that of group NC(P 0.01).The GIR60-120 in group HFE was significantly higher than that of group HFNE(P 0.01),and lower in group HFE than that of group NC(P 0.01).The serum PAI-1 in HF group increased significantly compared with NC group(P 0.01).The serum PAI-1 correlated positively with visceral fat mass(r =0.656,P 0.05).Compared with HFNE group,the serumsPAI-1 decreased significantly in group HFE(P 0.01) after 4 weeks of exercise.【Conclusions】High-fat-diet feed could induce insulin resistance rat's model.The mechanism of insulin resistance was relevant to elevated visceral fat mass.The mean level of the serum PAI-1 increased in insulin resistant rats,and the elevated PAI-1 level might be correlated with the visceral fat mass.A certain loading dose exercise could diminish the visceral fat mass and the content of serum PAI-1 and improved insulin sensitivity,which indicated that PAI-1 might be involved in the development and the progress of insulin resistance.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot