Title: Effect of hypothermia on inflammation after ischemic-reperfusion in rat
Abstract: Objective In this study we attempted to explore the neuroprotective effect of mild hypothermia against inflammatory cascade reaction after I/R in rat. Methods Adult male Sprague Dawley rats were randomly divided into sham-operated groups,controlled groups,and test groups. The ischemia-reperfusional model was established according to Longa’s method. In test groups animals continuously received head cooling during MCA occlusion and reperfusion,whereas in the controlled groups animals remained normal temperature.Results (1) The expression of TNF-α,IL-1β and ICAM-1 were up-regulated 2 hours after occlusion,and reached peak 6 to 12 hours after onset of ischemia. Mild hypothermia significantly suppressed the expression. (2) After 2 hours' occlusion the infiltrated PMNLs that mainly distributed around vessels could be seen in the infarct area. There appeared mass infiltrated PMNLs in peripheral infarct area after onset of ischemia 6 to 12 hours. Mild hypothermia significantly suppressed the infiltration of PMNLs. (3) No matter permanent MCAO groups or transient ischemia-reperfusional groups,was the effect of mild hypothermia evident. In the transient ischemia-reperfusional groups the animals that received 12 hours continuous head cooling had the least infarct volume. Conclusion The results described above suggest that inflammatory response plays an important role in I/R injury. Suppression on inflammatory cascade reaction after I/R may be one of the important neuroprotective mechanisms of mild hypothermia.
Publication Year: 2004
Publication Date: 2004-01-01
Language: en
Type: article
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