Title: Protective effect of intraventricle mild hypothermia on focal cerebral ischemia injury
Abstract: Objective To investigate the protective effect of intraventircle mild hypothermia on focal cerebral ischemia-reperfusion in rabbits and the neuroprotective molecule mechanism.Methods The middle cerebral artery occlusion (MCAO) and reperfusion models of rabbits were achieved by thread embolism.Eighty male rabbits were randomly divided into control group,occlusion group and mild hypothermia group.The neurofunction performance of Longa score and the edema volume of the brain by Elliott method were evaluated,the cells cyle and apoptosis rate were measured by flow cytometry (FCM) and the expression level of cysteinyl aspartate-specific protease-3 (Caspase-3) protein by Western blotting.Results The neurofunctional score in the mild hypothermia group was 2.167 ± 0.753,significantly lower than that in the occlusion group (P < 0.01).Dry-wet weight ratio in mild hypothermia group was 79.26 ± 1.30,which was significantly different from that in occlusion group and control group (P <0.01).Flow cytometry revealed that mild hypothermia significantly suppressed apoptosis rate (2.46 ± 0.84).The proportion of cells in G1 phase was declined to (64.05 ± 3.24),and that in S phase and G2 phase was increased to (43.79 ±2.25) and (12.88 ± 1.77) respectively (P <0.01).The hypothermia down-regulated Caspase317 kb expression (P <0.01).Conclusion The mild hypothermia could reduce brain edema,and inhibit apoptosis by down-regulating the Caspase-3 expression to protect the brain function.
Key words:
Mild hypothermia; Cerebral ischemia/reperfusion injure; Cysteinyl aspartate-specific protease-3 ; Apoptosis
Publication Year: 2012
Publication Date: 2012-12-08
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot