Title: The protection of low molecular heparin therapy on rat renal ischemia reperfusion injury:An experimental study
Abstract: Objective To approach the protection on rat renal ischemia reperfusion injury through regulating plasma levels of proinflammatory cytokines and antiinflammatory cytokines by low molecular weight heparin. Methods The model for rat renal ischemia reperfusion injury was established, 80 healthy Wistar rats were equally randomized into following groups: the blank control group, sham-operated control group, IRI control group and LMWH treatment group.Meanwhile,4 groups were stratified according to the postoperative observation periods (1h,3h,6h and 24h).Levels of serum creatinine (Scr), blood urea nitrogen (BUN), tumor necrosis factor-alpha(TNF-α),interleukin-6(IL-6),interleukin-4(IL-4)and interleukin-10(IL-10) were tested respectively in each group. Flow cytometry was used to examine the variation of intercellular adhesion molecular-1 (ICAM-1) of polymorphonuclear neutrophils (PMNs). Results (1)After reperfusion, plasma levels of IL-4, IL-10 were reduced, and levels of TNF-α, IL-6, ICAM-1 were significantly increased when compared with that of the sham-operated control group. The three increase spots of peak value were similar to each other. Correlation analysis showed that there was a significant positive correlation between ICAM-1 and IL-6.And also IL-6 correlated with TNFα. (2)After LMWH treatment, plasma levels of TNF-α, IL-6 were declined while IL-4, IL-10 rised. The expressions of ICAM-1 in PMNs were significantly decreased when compared with that of the IRI control group (P0.05). Conclusions (1)IRI may cause the increase of proinflammatory cytokine in rat plasma, activate ICAM-1 to coordinate immunoreaction between leucocyte and renal tubular epithelial cell, and lead to AKI; (2)LMWH may accommodate levels of proinflammatory cytokines and antiinflammatory cytokines to keep the equilibrium state, inhibit the expression of ICAM-1, and benefit for rebuilding the steady state of immune system to protect the renal function.
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
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