Title: Study on Changes and Significance of Proinflammatory Cytokines in Serum and Real Tissues of Rats with Renal Ischemia-reperfusion Injury
Abstract: Objective:To explore the effect of proinflammatory cytokines interleukin-6 (IL-6), IL-8, tumor necrosis factor-α (TNF-α)in rats with renal ischemia-reperfusion injury (IRI). Methods: The model of renal IRI was replicated by closing renal artery and vein in rat and the levels of IL-6, IL-8, TNF-α in renal tissues and serum were determined by enzyme-linked immunosorbent assay (ELISA). The renal coefficients, serum urea nitrogen (SUN) and serum creatinine (SCr) were also measured. Results:The levels of IL-6 in renal tissues in pure ischemia group and ischemia reperfusion 4 (IR4 h) and 24 h hour (IR24 h) groups were lower than that in control group (P0. 05) ,and the levels of IL-6 in serum only in pure ischemia group was lower than that in control group. Linear positive correlation was found between blood and renal tissues IL-6 concentrations in different stages (P0. 05). The levels of IL-8,TNF-α in renal tissues in pure ischemia and IR1 h,IR4 h groups were higher than that in control group (P0. 01 ,0. 05) ,and the levels of IL-8 in serum in pure ischemia and IR1 h groups were lower than that in control group (P0. 01). The levels of SUN, SCr and renal coefficients in every group of ischemia-reperfusion were higher than that in control group. Conclusion: IL-8, TNF-α might play an important role in early renal IRI. IL-6 might become favourable effect in different stages of renal IRI.
Publication Year: 2005
Publication Date: 2005-01-01
Language: en
Type: article
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