Title: Germplasm Screening and Hybrid Rice Purity Testing with Functional Marker of Wide Compatibility Gene S5-n
Abstract: In order to improve the precision of marker-assisted selection for S5-n,a functional marker S5136 based on the 136 bp deletion in the DNA sequence of S5-n was developed.The sterile line Yuetai A with S5-n has been identified with marker S5136,and conformed by sequencing.There was a SNP in the downstream of the 136 bp DNA deletion region of Yuetai A,which has been discovered in the wide compatible germplasm resources from India.The polymorphism was identified with marker S5136 between the photo-thermo sensitive sterile line Peiai 64S and the parent R986.Therefore S5136 was a perfect marker to test the purity of commercial hybrid seed of Liangyou 986(Peiai64s/R986).Four Peiai 64S plants were identified from the 233 sampled plants with S5136,with self seed setting rate of Peiai64S being 1.72%.The PCR efficiency was compared between the SDS DNA extracting method and the simple-way DNA extracting method.The results showed that the DNA extracted by the simple-way was better,except that the DNA could not be kept for more than 2 weeks at room temperature.Therefore,the functional marker S5136 was a powerful molecular marker in S5-n germplasm screening and seed purity testing for the two line hybrids with Peiai 64S as maternal parent.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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Cited By Count: 3
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