Title: Construction of Fingerprint and Identification for Foux Processing Tomato Hybrids by SSR Marker
Abstract: [Objective]In order to identify the genetic diversity of tomato hybrids and establish the identification method for seed purity.[Method]Four processing tomato hybrids and eight parent lines were screened for simple sequence repeat(SSR) primers.Gel electrophoresis of the amplified product was carried out with 4% polyacrylamide and further study on their polymorphmism.[Result]Among 34 pairs of primers,10 pairs of steady polymorphism were selected and distributed in tomato genome.2 to 4 alleles were detected by each pair of primers.There were 28 polymorphism fragments in total,whose average number was 2.8 per pair of primer.The fragment size ranged from 60 to 150bp.And DNA fingerprinting of successfully constructed.In addition,codominant markers of the four hybrids were identified.[Conclusion]These markers not only can be used to distinguish four processing tomato hybrids and eight parent lines between real and false parent materials or hybrids,but also to supply reliable methods for hybrids seed purity inspection,which is of great importance for the property protection of the parent lines and safe popularization of hybrid seeds.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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