Title: Study on Xylanase(xylB) Gene from Bacillus brevi
Abstract:In this study,a pairs of primers were designed according to the published nucleotide sequences of putative xylanase genes of Bacillus circulan and Bacillus subtilis.With the specific primers,a target ...In this study,a pairs of primers were designed according to the published nucleotide sequences of putative xylanase genes of Bacillus circulan and Bacillus subtilis.With the specific primers,a target fragment of the β-1,4-endo-xylanase gene was firstly amplified from Bacillus brevi L8.Sequence analyses showed the homology of the cloned gene to AF490979.1and AF490980.1 were 97% and 96%,respectively.The recombinant expression plasmid was constructed and transformed into E.coli BL21(DE3).SDS-PAGE demonstrated that the β-1,4-endo-xylanase protein gene were expressed extracellularly in E.coli BL21(DE3) successfully.A maximum activity of 26.14 U/mL achieved gotten from cellular extract of pET-30a-xylB induced by IPTG.The optimum pH and temperature of the purified enzyme were 9.0 and 50 ℃,respectively.Read More
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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