Title: Study on expression of MMP-9 and VEGF in lung adenocarcinoma
Abstract: Objective To investigate the correlation between matrix metalloprotoinase-9(MMP-9)and vascular endothelial growth factor(VEGF)expressions in lung adenocarcinoma and its significance.Methods The immunohistochemistry(SP)was used to test the expressions of MMP-9and VEGF in 30 specimens of lung adenocarcinoma and 12 specimens of normal paracancerous lung tissues.Western blot was used to detect the change of VEGF protein levels in lung adenocarcinoma A549 cells after blocking MMP-9by MMP-9mAb,and the change of MMP-9protein levels in lung adenocarcinoma A549 cells after blocking VEGF by VEGF mAb.Results The high expression rate of MMP-9protein in lung adenocarcinoma tissues was 63.3%,which in the normal tissues was 16.7%,the difference had statistical significance(P0.05);the high expression rate of VEGF protein in lung adenocarcinoma tissues was70%,which in the normal tissues was 25%,the difference had statistical significance(P0.05);the high expression rate of MMP-9in the lung adenocarcinoma was positively correlated with the high expression rate of VEGF(r=0.709 4,P0.01),and which was correlated with the lymph node metastasis status and the clinical TNM stage(P0.05).The high expression rate of VEGF was correlated with the lymph node metastasis status and the clinical TNM stage(P0.05).Blocking MMP-9by MMP-9mAb could reduce the VEGF protein expression level.Blocking VEGF by VEGF mAb could reduce the MMP-9protein expression level.Conclusion VEGF and MMP-9are highly expressed in lung adenocarcinoma and have the synergistic action in promoting invasion and metastasis of lung adenocarcinoma.
Publication Year: 2015
Publication Date: 2015-01-01
Language: en
Type: article
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