Title: Immune responses induced by intranasal immunization with inactivated influenza A split virus-antibody complex in mice
Abstract: Objective To study the immune responses induced by immuno-stimulating complexes in respiratory virus infections. Methods Inactivated influenza A (H3N2) split-virus complexed to its antibody was used for intranasal immunization of BALB/c mice. H3N2-CpG, H3N2-PEG or H3N2 alone were separately used as control immunogens. Tracheal-lung wash fluid and serum samples were assayed for anti-H3N2 IgA and IgG respectively. Splenocytes were stimulated with H3N2 hemagglutinin and assayed for interferon-γand interleukin-4 in cell culture supernatants. Results H3N2-antibody complex could induce IgA antibody in lung wash fluid at a level similar to that induced by H3N2-CpG. The titers of IgA of both groups were significantly higher than that induced by H3N2 alone (P0.01) . In addition, high level of interferon-γwas detected in the supernatant of cultured splenocytes in H3N2-CpG immunized mice. Conclusion H3N2-antibody complex and H3N2-CpG can induce potent mucosal and humoral immunity. CpG DNA show strong Th1-type adjuvant activity for H3N2 antigen specific responses.
Publication Year: 2006
Publication Date: 2006-01-01
Language: en
Type: article
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