Title: Determination of Paeniflorin Content in Lidan Zhitong Capsules by HPLC
Abstract: Objective:To establish a HPLC method for determining paeoniflorin content in Lidan Zhitong capsules.Method: The content of paeoniflorin from Radix paeoniad rubra was idetermined by HPLC.The Agilent Extend-C18 column(4 mm×250 mm,5 μm) was used with acetonitrile-0.1% phosphoric acid solution(15∶85) as mobile phase;the detection wavelength was set at 230 nm;flow rate was 1 mL·min-1.Number of theoretical plates by paeoniflorin peak calculation should not be less than 4 000.Result: The calibration curve of paeoniflorin was linear in the range of 0.144-0.720 μg.RSD of precision was 0.54%.RSD of stability was 1.17%.RSD of reproducibility was 1.48%.The average recovery was 97.77% with RSD of 1.48%.Conclusion: The method is selective and sensitive,can be used for quality of control of Lidan Zhitong capsule.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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