Title: Methodological for determining yield of Gentiooligsaccharide prepared by enzyme
Abstract: In order to determine yield of Gentiooligsaccharide quantitatively when it was prepared by enzyme,the Oxidase-Peroxidase(GOD-POD) method was used to determine content of residual substrate glucose,and to measure yield of Gentiooligsaccharide indirectly.The interference factors of GOD-POD method for determining content of residual substrate glucose in system of Gentiooligsaccharide prepared by enzyme,the stability,repeatability,and precision of the method were all studied.It was showed the results of determining content of residual substrate glucose by GOD-POD method were stable in two hours(RSD=0.81%),with good repeatability(RSD=0.97%),high precision(RSD=1.55%),and slight inference(the average recovery=101.681%),indicating that the results of determination yield of Gentiooligsaccharide were stable and trustworthy.Gentiooligsaccharide and other kinds of materials did not affect the determination notably.It was available to use GOD-POD method to determine indirectly yield of Gentiooligsaccharide prepared by enzyme.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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