Title: Significance of p53 and K-ras Gene Mutation of Peripheral Blood in Diagnosis of Gastric Carcinoma
Abstract: Objective To investigate clinical significance of p53 and K-ras mutation of peripheral blood in patients with gastric carcinoma.Methods Totally 73 patients with gastric carcinoma were chosen to detect gene mutation of p53 and K-ras with PCR-SSCP technology,and another 20 cases of non-tumor patients were enrolled as the controls.Results Among 73 patients,there were 7 who have p53 mutation in peripheral blood,with positivity rate of 9.6%,and 5 who have K-ras mutation,with a positivity rate of 6.8%.No p53 and K-ras mutation was found in peripheral blood of the controls.There was a significant difference in positivity rate of the p53 and K-ras mutation between the patients who have liver metastasis and who have not (P0.05),however,there was no significant difference in positivity rate of the p53 and K-ras mutation between the patients with different cancer grades of differentiation and clinicopathologic stages (P0.05).Conclusion Detection of gene mutation of cancer cell in peripheral blood by PCR-SSCP has a certain value to early diagnosis of hepatic micrometastasis in gastric carcinoma.
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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