Title: The in vitro and in vivo chemotactic effect on immune cells by hMIP-1β gene transfected murine colorectal adenocarcinoma CT26 cells
Abstract: This study was to investigate the changes of biological characters and tumorigenicity and the chemotactic activities to immune cells of tumor cells transfected with human macrophage inflammatory protein-1 beta(hMIP-1β) gene.Murine colorectal adenocarcinoma CT26 cells were transfected with a recombinant adenovirus carrying the hMIP-1β gene(AdhMIP-1β).Then the gene transfected was tested by X-gal method and hMIP-1β level in supernatants of hMIP-1β transfected CT26 cells was assayed by ELISA.The chemotactic activity for CD4~(+) T cells,CD8~(+) T cells,NK cells and immature dendritic cells(imDC) were assayed by a transwell chamber,and change of growth characterization and in vivo tumorigenicity of hMIP-1β gene-transfected CT26 cells were also investigated.The results showed that hMIP1β gene could be transfected into CT26 cells by AdhMIP-1β efficienctly.The level of hMIP-1β in the culture supernatant of hMIP-1β gene-transfected CT26 cells was enhanced and the supernatant displayed marked chemotactic activities to CD4~(+) T cells,CD8~(+) T cells,NK cells and imDC compared with LacZ gene-transfected CT26 cells and control.When the hMIP-1β gene-transfected CT26 cells were subcutaneously inoculated to BALB/c mice,tumorigencity was delayed and suppressed,and overt necrosis and lymphocytes infiltration were observed in tumor tissue but not in those inoculated with LacZ gene-transfected CT26 cells or parental CT26 cells.These findings implicate that hMIP-1β gene could be transfected into CT26 cells and be expressed by AdhMIP-1β with high efficiency,and hMIP-1β gene-transfected CT26 cells exhibited decreased tumorigenicity and could induce antitumor immunity.The data provided a basis for further investigation on tumor vaccine transfected with hMIP-1β gene.
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
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