Title: Effect of lung cancer cells transfected with interleukin 18 gene on the immunological activity of dendritic cells
Abstract: AIM: To study the effect of interleukin 18 gene transfected lung cancer cells on the phenotype and immunological activity of dendritic cells(DC).METHODS: A secretive IL-18 expression vector containing IL-12 P40 signal sequence was constructed and transfected into NCI-H460 lung cancer cells.DC induced from human peripheral blood were divided into 4 groups(NT,PV,GT and PD).DC were stimulated by non-transfected NCI-H460 cells,pure vector transfected NCI-H460 cells and IL-18 transfected NCI-H460 cells respectively for group NT,PV,GT,and non-stimulated DC for group PD.CD54,CD80,CD83 and CD86 on DC in the 4 groups were detected with flow cytometry.T cell proliferation stimulated by DC in the 4 groups was assayed with MTT method.IL-12 release in cultured DC supernatant was measured by ELISA.RESULTS: Sequencing result of the secretive IL-18 was correct.The transfected cells expressed IL-18 fusion gene and 18 kD IL-18 protein.DC in GT group expressed more surface molecules than those in other 3 groups.T cell proliferation and IL-12 secretion in GT group were higher than those in other 3 groups.CONCLUSION: IL-18 gene transfected NCI-H460 cell increases surface molecule expressions on DC.It also enhances immunological activity and IL-12 secretion in DC.
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
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