Title: Protective effects of a new ROS scavenger on the retinal cells after optical nerve crash in rats
Abstract: Objective To investigate the content of reactive oxygen species(ROS)during retinal cells apoptosis after optical nerve crash in rats and edaravone's protective effects on retinal cells.Methods A total of 192 Sprague-Dawley rats(96 males and 96 females)were randomly divided into normal group,sham group,control group and treatment group,with48 rats in each group.In the control and the treatment group,the model of SD rat optic nerve crush was made with optic nerve crush method.In the treatment group,edaravone(30 mg/kg)diluted with normal saline was injected intraperitoneally after modeling every 12 hours with delivery time of 30 minutes for 14 days.The rats were killed at day 3,6,10 and 14 of the experiment.2',7'-dichloro fluorescein pair of dihydroartemisinin acetate ester(DCFH-DA)was used as the labeled probe;fluorescence intensity of 2',7'-dichloro fluorescein dihydrotestosterone(DCF)in retinal cells was checked by flow cytometry at different time points in the 4 groups.Apoptosis rate of the retinal cells was checked by double staining of AnnexinV/PI via flow cytometry at different time points in the 4 groups.Morphology of cell apoptosis was observed with laser confocal microscope. The ultrastructural changes of retinal ganglion cells(RGCs)were detected by TEM.Results At 3 d,6 d,10 d and 14 d,the expression of ROS and apoptosis rate of retinal cells in the control group were significantly increased at each time point compared with those in the treatment group and the normal group(P0.05).There were no significant differences in all indexes between sham group and the normal group.Cellular damage in the treatment group abated obviously compared with that in the control group.Conclusion Edaravone prevents the apoptosis of retinal cells by eliminating ROS and thus has a protective effect on retinal cells.
Publication Year: 2014
Publication Date: 2014-01-01
Language: en
Type: article
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