Title: The effects of VEGF on proliferation and migration of cultured human umbilical vein endothelial cells
Abstract: Objective To study the effects of VEGF secreted by hypoxia-cultured cardiomyocytes on proliferation and migration of cultured human umbilical vein endothelial cells (HUVECs).Methods HUVECs were cultured and divided into 4 groups:normal control,hypoxia group,the group cultured in the medium containing VEGF secreted by hypoxia-cultured cardiomyocytes,VEGF+hypoxia group.The proliferation and migration of HUVECs were assayed as follows:cell cycle by flow cytometry,proliferating cell nuclear antigen (PCNA) by immunohistochemistry,protein synthesis by 3H-leucine incorporation,and the numbers of migratory cells were counted under microscope.Results VEGF accelerated the change of HUVECs from G 0/G 1 to S phases and increased the numbers of cells of S and G 2/M phases,the expression of PCNA, 3H-leucine incorporation and the numbers of migratory cell in normal and hypoxia cultures.Hypoxia inhibited the process.VEGF antagonized the effects of hypoxia.Conclusion The VEGF secreted by hypoxia-cultured cardiomyocytes could increase HUVECs proliferation and migration,and reverse the inhibitory action of hypoxia.
Publication Year: 2004
Publication Date: 2004-01-01
Language: en
Type: article
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