Title: IL-12 Retroviral Expression Vector pL35P40SN Transfected B Cell as a Adjuvant for Tumor Vaccine
Abstract: Objective To transfect B cell with recombinant human IL-12 retroviral expression vector pL35P40SN,and to identify its influence on Th cell differentiation and CTL function.Methods We transfect B cell with pL35 P40SN,detect IL-12 production in the cells supernatant with ELISA method,detect the transfected B cell's influence on Th cell differentiatinn and CTL function,with IFN-γ,production and ~3H-TdR method respectively.Results When transfeeted B cell with recombinant human IL-12 retroviral expression vector pL35P40SN,There were more IL-12 produc- tion in the cells supernatant based on ELISA detection,there were more IFN-γproduction in the Th ceils supernatant, CTL function were more stronger then the pLXPXSN transfected B cell.Conclusion pL35P40SN transfected B cell can promote Th_1 cell differentiation and CTL function.
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
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