Title: Induction of specific antitumor immunity by human dendritic cells transfected with recombinant human eukaryotic vector pMAGE-A1/EGFP
Abstract: Objective:To induce MAGE-A1specific T cell immunity by dendritic cells(DCs) transfected with recombinant human eukaryotic vector pMAGE-Al/EGFP. Methods:The recombinant human eukaryotic vector pMAGE-Al/EGFP was constructed and transfected into human PBMC-derived DCs. Using FACS analysis and intracellular staining methods,differences in proteins expression, maturation and capacity to induce MAGE-A1 specific T cell immunity of DCs before and after transfection were identified. Results:Successfully the recombinant human eukaryotic vector pMAGE-Al/EGFP was constructed and equally MAGE-A1 and EGFP was expressed after transfection into DCs by electroporation, which was 8. 8% ± 0. 9% and 8. 47% ± 0.78% respectively. After transfection,the expression of CD86,CD40L and CD83 were upregulated,which suggested that maturation of DCs was enhanced. MAGE-A1 expressed on the surface of transfected DCs could be combined with anti-MAGE-A1 monoclonal antibody specially, and indued amplification of MAGE-A1 specific CD4+T cell clones,which secreted more IFN-γ when contacting with MAGE-A1 antigen once again. Conclusion: As a kind of promising vaccine, DCs transfected with human eukaryotic vector pMAGE-A1/EGFP could not only induce MAGE-A1 specific T cell immunity in vitro,but also be detected by FACS rapidly as well,thus will give hope to immunotherapy based on MAGE-A1.
Publication Year: 2006
Publication Date: 2006-01-01
Language: en
Type: article
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