Title: Experimental study of the effect of endostatin on the proliferation and metastasis of human colon cancer xenograft in nude mice
Abstract: Purpose To investigate the effect of angiogenesis inhibitor (endostatin ) on the proliferation and metastasis of colon cancers in vivo. To explore the role of matrix metalloproteinase-13(MMP-13) on angiogenesis in colon cancers. Methods Metastatic model of human colon cancer was established by implanting histological intact human tumor tissue into colon wall of nude mice. Endostatin was administered every day for six weeks at dose of 0 mg/kg,5 mg/kg,10 mg/kg,20 mg/kg. Seven weeks after implantation,the nude mice were sacrificed. The tumor volume,inhibition rates,matrix metalloproteinase-13 (MMP-13),intratumoral microvessel density (MVD) and the presence of metastases are evaluated.Results Compared with the untreated controls ,proliferation of the orthotopically implanted tumor was significantly reduced in size in nude mice treated with endostatin. Administered at the dosage of 0 mg/kg,5 mg/kg,10 mg/kg,20 mg/kg,inhibition rates were 0,62.0%,78.2% and 84.5% respectively. MMP-13 was 81.8%,58.3%,42.9%,21.4%;MVD was 11.20±3.9,6.1±2.6,2.4±1.6,1.22±0.4;The rates of peritoneal metastasis were 81.8 %,50.0%,28.5%,7.1%;The rates of liver metastasis were 63.6%,33.3%,14.3%,0. Conclusions MMP-13 might be one of the important factors in the angiogenesis of colon cancers. Angiogenesis inhibitor,endostatin can reduce the tumor size by inhibiting tumor angiogenesis,and has inhibitory effect both on tumor proliferation and metastasis of human colon cancer xenograft in nude mice.
Publication Year: 2005
Publication Date: 2005-01-01
Language: en
Type: article
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Cited By Count: 1
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